DETECTION OF POLIOVIRUS BY CELL-CULTURE AND BY PCR AFTER UV DISINFECTION

被引：13

作者：

MAIER, A

TOUGIANIDOU, D

WIEDENMANN, A

BOTZENHART, K

机构：

[1] Hygiene-lnstitut der Eberhard-Karls-Universität Abteilung Allgemeine Hygiene und Umwelthygiene, Tübingen, 72074

来源：

WATER SCIENCE AND TECHNOLOGY | 1995年 / 31卷 / 5-6期

关键词：

POLIOVIRUS; UV IRRADIATION; DISINFECTION; CELL CULTURE; PCR; SEMI-NESTED PCR;

D O I：

10.1016/0273-1223(95)00255-L

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The polymerase chain reaction (PCR) is used for the detection of microrganisms in water, but it is known that one does not detect only viable microorganisms by PCR. The following results show the detection of poliovirus on a cell culture assay and by PCR after inactivation by UV irradiation. The virus was irradiated with a low pressure mercury lamp with times up to 4000 seconds (=3980 J/m(2)). A 4 log inactivation of the virus was complete after 300 seconds (=294 J/m(2)) as shown by the cell culture assay. The viral RNA was detectable by PCR even at irradiation times of 4000 seconds.

引用

页码：141 / 145

页数：5