A VECTOR FOR THE SITE-DIRECTED, GENOMIC INTEGRATION OF FOREIGN DNA INTO SOYBEAN ROOT-NODULE BACTERIA

被引：18

作者：

ACUNA, G ^{[1
]}

ALVAREZMORALES, A ^{[1
]}

HAHN, M ^{[1
]}

HENNECKE, H ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL, INST MIKROBIOL, UNIV STR 2, CH-8092 ZURICH, SWITZERLAND

来源：

PLANT MOLECULAR BIOLOGY | 1987年 / 9卷 / 01期

关键词：

D O I：

10.1007/BF00017985

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A non-essential DNA region carrying two different repeated sequences (RS.beta.3 and RS.alpha.9) adjacent to a nitrogen fixation (nif) gene cluster has been identified previously in Bradyrhizobium japonicum strain 110. In closely related B. japonicum strains a similar genomic arrangement was found. We constructed a mobilizable plasmid vector carrying RS.beta.3 and RS.alpha.9, and a kanamycin resistance cassette (nptII gene) plus suitable cloning sites inserted between the two repeated sequences. Using this vector (pRJ1035), stable integration of a lacZ gene fusion into the B. japonicum genomic RS region was achieved. The resulting strain yielded more than 10-fold higher .beta.-galactosidase activity in soybean root nodules as compared to a B. japonicum strain carrying the same lacZ fusion on a pRK290-based plasmid.

引用

页码：41 / 50

页数：10

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