WHOLE-CELL PATCH-CLAMP RECORDINGS OF AN NMDA RECEPTOR-MEDIATED SYNAPTIC CURRENT IN RAT HIPPOCAMPAL SLICES

被引：38

作者：

RANDALL, AD ^{[1
]}

SCHOFIELD, JG ^{[1
]}

COLLINGRIDGE, GL ^{[1
]}

机构：

[1] UNIV BRISTOL, SCH MED SCI, DEPT PHARMACOL, BRISTOL BS8 1TH, AVON, ENGLAND

来源：

NEUROSCIENCE LETTERS | 1990年 / 114卷 / 02期

基金：

英国惠康基金;

关键词：

6-Cyano-7-nitroquinoxaline-2,3-dione; d-2-Amino-5-phosphonovalerate; Hippocampus; N-Methyl-d-aspartate receptor; Picrotoxin; Synaptic current;

D O I：

10.1016/0304-3940(90)90070-P

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Whole-cell patch-clamp recordings and pharmacological techniques have been used to obtain low noise recordings of 2 excitatory postsynaptic synaptic currents (termed EPSAA and EPSCB) evoked by stimulation of the Schaffer collateral-commissural pathway in rat hippocampal slices. EPSCA was blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and EPSCB was blocked by d-2-amino-5-phosphonovalerate (APV), indicating their mediation by non-N-methyl-d-aspartate (non-NMDA) and NMDA receptors, respectively. EPSCB has a slower time-course than EPSCA and its current-voltage relationship was highly non-linear with a region of negative slope conductance from -35 to -100 mV. These properties of EPSCA and EPSCB can explain their differing participation in synaptic transmission in this pathway. © 1990.

引用

页码：191 / 196

页数：6

共 20 条

[1] EFFECTS OF NEW NON-N-METHYL-D-ASPARTATE ANTAGONISTS ON SYNAPTIC TRANSMISSION IN THE INVITRO RAT HIPPOCAMPUS
ANDREASEN, M
LAMBERT, JDC
JENSEN, MS
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1989, 414 : 317 - 336
[2] ASCHER P, 1988, J PHYSIOL-LONDON, V399, P247
[3] WHOLE CELL RECORDING FROM NEURONS IN SLICES OF REPTILIAN AND MAMMALIAN CEREBRAL-CORTEX
BLANTON, MG
LOTURCO, JJ
KRIEGSTEIN, AR
[J]. JOURNAL OF NEUROSCIENCE METHODS, 1989, 30 (03) : 203 - 210
[4] MECHANISM OF ANION PERMEATION THROUGH CHANNELS GATED BY GLYCINE AND GAMMA-AMINOBUTYRIC-ACID IN MOUSE CULTURED SPINAL NEURONS
BORMANN, J
HAMILL, OP
SAKMANN, B
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1987, 385 : 243 - 286
[5] COLLINGRIDGE GL, 1988, J PHYSIOL-LONDON, V399, P283
[6] INHIBITORY POST-SYNAPTIC CURRENTS IN RAT HIPPOCAMPAL CA1 NEURONS
COLLINGRIDGE, GL
GAGE, PW
ROBERTSON, B
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1984, 356 (NOV): : 551 - 564
[7] EXCITATORY AMINO-ACIDS IN SYNAPTIC TRANSMISSION IN THE SCHAFFER COLLATERAL COMMISSURAL PATHWAY OF THE RAT HIPPOCAMPUS
COLLINGRIDGE, GL
KEHL, SJ
MCLENNAN, H
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1983, 334 (JAN): : 33 - 46
[8] DUAL-COMPONENT AMINO-ACID-MEDIATED SYNAPTIC POTENTIALS - EXCITATORY DRIVE FOR SWIMMING IN XENOPUS EMBRYOS
DALE, N
ROBERTS, A
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1985, 363 (JUN): : 35 - 59
[9] PAIRED-PULSE DEPRESSION OF MONOSYNAPTIC GABA-MEDIATED INHIBITORY POSTSYNAPTIC RESPONSES IN RAT HIPPOCAMPUS
DAVIES, CH
DAVIES, SN
COLLINGRIDGE, GL
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1990, 424 : 513 - 531
[10] GAMMA-AMINOBUTYRIC ACID UPTAKE AND THE TERMINATION OF INHIBITORY SYNAPTIC POTENTIALS IN THE RAT HIPPOCAMPAL SLICE
DINGLEDINE, R
KORN, SJ
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1985, 366 (SEP): : 387 - 409

← 1 2 →