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GENOMIC ANALYSIS USING A YEAST ARTIFICIAL CHROMOSOME LIBRARY WITH MOUSE DNA INSERTS

被引：55
作者：
ROSSI, JM
BURKE, DT
LEUNG, JCM
KOOS, DS
CHEN, HC
TILGHMAN, SM
机构：
[1] PRINCETON UNIV, HOWARD HUGHES MED INST, PRINCETON, NJ 08544 USA
[2] PRINCETON UNIV, DEPT MOLEC BIOL, PRINCETON, NJ 08544 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 06期
关键词：
CHROMOSOME WALKING; FU LOCUS; PHYSICAL MAPPING; MOUSE GENETICS;
D O I：
10.1073/pnas.89.6.2456
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.
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页码：2456 / 2460
页数：5
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