A MICROASSAY FOR PROTEOLYTIC ACTIVITY

被引：31

作者：

PLANTNER, JJ

机构：

[1] Lorand V. Johnson Laboratory for Eye Research, Department of Surgery, Division of Ophthalmology, Cleveland

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90307-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A quantitative procedure for measuring proteolytic activity, utilizing azoalbumin as substrate, has been developed for use in microtiter plates. An enzyme-linked immunosorbent assay reader is used to measure absorbance. The procedure is sensitive, as well as being both rapid and economical. It is particularly convenient for measuring large numbers of samples, such as fractions from column chromatography. © 1991.

引用

页码：129 / 131

页数：3

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