DIFFERENCES IN TEMPERATURE-SENSITIVE RECEPTOR-BINDING OF GLUCOCORTICOIDS IN SPONTANEOUSLY HYPERTENSIVE AND NORMOTENSIVE WISTAR-KYOTO RATS

被引:10
作者
PANARELLI, M [1 ]
HOLLOWAY, CD [1 ]
BARR, ABP [1 ]
FRASER, R [1 ]
KENYON, CJ [1 ]
机构
[1] WESTERN INFIRM & ASSOCIATED HOSP,MRC,BLOOD PRESSURE UNIT,GLASGOW G11 6NT,LANARK,SCOTLAND
关键词
GLUCOCORTICOID; HYPERTENSION; RAT; RECEPTOR;
D O I
10.1016/0039-128X(94)00011-Z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Glucocorticoid receptor binding was compared in liver cytosol preparations from spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats using homologous displacement of [H-3]dexamethasone. At 5 degrees C, there was no difference in receptor binding affinity or concentration between strains for dexamethasone, corticosterone or aldosterone. At 37 degrees C, affinity for dexamethasone was lower than at 5 degrees C for both rat strains and decreased with time. However, al this higher temperature, binding affinity in the SHR preparation was consistently higher than in the WKY preparation. The WKY preparation had a higher receptor concentration. The rate of dissociation of the [H-3]dexamethasone-receptor complex prepared at 5 degrees C and then incubated at 37 degrees C was rapid but not different between strains. A possible explanation of these results is that the relationship of the heat shock proteins to the receptor heterocomplex is different between strains. Evidence exists of a genetic difference in Hsp 70 between SHR and WKY rats, although its cosegregation with blood pressure has not been established.
引用
收藏
页码:73 / 75
页数:3
相关论文
共 15 条
[1]  
BROWNIE AC, 1990, ENDOCRINE HYPERTENSI, P29
[2]   EVIDENCE OF ABNORMALITIES IN CORTICOSTEROID SECRETION LEADING TO VOLUME-DEPENDENT HYPERTENSION IN MILAN RATS [J].
FRASER, R ;
ANCIL, AK ;
BROWN, WB ;
INGRAM, MC ;
HOLLOWAY, CD ;
HENDERSON, IW ;
KENYON, CJ .
HYPERTENSION, 1994, 24 (04) :512-515
[3]   RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF HSP70-GENE, LOCALIZED IN THE RT1-COMPLEX, IS ASSOCIATED WITH HYPERTENSION IN SPONTANEOUSLY HYPERTENSIVE RATS [J].
HAMET, P ;
KONG, D ;
PRAVENEC, M ;
KUNES, J ;
KREN, V ;
KLIR, P ;
SUN, YL ;
TREMBLAY, J .
HYPERTENSION, 1992, 19 (06) :611-614
[4]   THE ROLE OF ALDOSTERONE IN THE DEVELOPMENT OF HYPERTENSION IN SPONTANEOUSLY HYPERTENSIVE RATS [J].
KENYON, CJ ;
DECONTI, GA ;
CUPOLO, NA ;
MORRIS, DJ .
ENDOCRINOLOGY, 1981, 109 (06) :1841-1845
[5]   THE ROLE OF GLUCOCORTICOID ACTIVITY IN THE INHERITANCE OF HYPERTENSION - STUDIES IN THE RAT [J].
KENYON, CJ ;
PANARELLI, M ;
HOLLOWAY, CD ;
DUNLOP, D ;
MORTON, JJ ;
CONNELL, JMC ;
FRASER, R .
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1993, 45 (1-3) :7-11
[6]  
LAUX M, 1989, J STEROID BIOCHEM, V36, P331
[7]   FAILURE OF THE HEAT-SHOCK PROTEIN-70 LOCUS TO COSEGREGATE WITH BLOOD-PRESSURE IN SPONTANEOUSLY HYPERTENSIVE RAT X WISTAR-KYOTO RAT CROSS [J].
LODWICK, D ;
KAISER, MA ;
HARRIS, J ;
PRIVAT, P ;
VINCENT, M ;
SASSARD, J ;
SAMANI, NJ .
JOURNAL OF HYPERTENSION, 1993, 11 (10) :1047-1051
[8]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[9]   THERMOSENSITIVITY, A POSSIBLE NEW LOCUS INVOLVED IN GENETIC-HYPERTENSION [J].
MALO, D ;
SCHLAGER, G ;
TREMBLAY, J ;
HAMET, P .
HYPERTENSION, 1989, 14 (02) :121-128
[10]  
MANTERO F, 1983, J HYPERTENSION S2, V1, P150