FORMATION OF 8-NITROGUANINE IN DNA TREATED WITH PEROXYNITRITE IN-VITRO AND ITS RAPID REMOVAL FROM DNA BY DEPURINATION

被引：290

作者：

YERMILOV, V ^{[1
]}

RUBIO, J ^{[1
]}

OHSHIMA, H ^{[1
]}

机构：

[1] INT AGCY RES CANC, ENDOGENOUS CANC RISK FACTORS UNIT, F-69372 LYON 03, FRANCE

来源：

FEBS LETTERS | 1995年 / 376卷 / 03期

关键词：

PEROXYNITRITE; 8-NITROGUANINE; DNA DAMAGE; 8-OXOGUANINE; NITRIC OXIDE; SUPEROXIDE;

D O I：

10.1016/0014-5793(95)01281-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peroxynitrite is a strong oxidant formed by reaction of nitric oxide with superoxide in inflamed tissues. We have demonstrated that 8-nitroguanine is formed dose-dependently in calf thymus DNA incubated with low concentrations of peroxynitrite in vitro. 8-Nitroguanine in acid-hydrolyzed DNA was chemically reduced into 8-aminoguanine, which was analyzed using high performance liquid chromatography with electrochemical detection. Only peroxynitrite, but not nitrite, tetranitromethane nor NO-releasing compounds, formed 8-nitroguanine. Antioxidants and desferrioxamine inhibited the reaction. 8-Nitroguanine was depurinated from DNA incubated at pH 7.4, 37 degrees C (t(1/2) = similar to 4 h). Peroxynitrite did not increase 8-oxoguanine levels in DNA.

引用

页码：207 / 210

页数：4

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