ENDOGENOUS PROSTAGLANDIN-E(2) MODULATES CALCIUM-INDUCED DIFFERENTIATION IN HUMAN SKIN KERATINOCYTES

被引:40
作者
EVANS, CB
PILLAI, S
GOLDYNE, ME
机构
[1] UNIV CALIF SAN FRANCISCO,VET ADM MED CTR,DEPT DERMATOL,SAN FRANCISCO,CA 94121
[2] UNIV CALIF SAN FRANCISCO,VET ADM MED CTR,DEPT MED,SAN FRANCISCO,CA 94121
来源
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1993年 / 49卷 / 04期
关键词
D O I
10.1016/0952-3278(93)90025-R
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The concentration of extracellular calcium appears critical to the initiation of keratinocyte differentiation. Prostaglandins (PGs) have also been implicated in cell differentiation. Consequently, the participation of endogenous eicosanoids in calcium-induced differentiation of human keratinocytes was evaluated in vitro. Our results demonstrate that: (1) exogenously introduced PGE2, the major keratinocyte-derived eicosanoid, but not prostaglandin I2 (PGI2) or its stable metabolite 6-keto-PGF1alpha, enhances calcium-induced cornified envelope formation, an established marker of keratinocyte differentiation; (2) increasing extracellular calcium increased endogenous PGE2 synthesis by cultured keratinocytes, (3) blocking endogenous PGE2 synthesis with indomethacin significantly suppresses calcium-induced formation of the cornified envelope; and (4) adding back PGE2 to indomethacin-treated keratinocytes is able to re-establish the control level of cornified envelope formation following stimulation by calcium. These data document the participation of endogenously generated PGE2 in the modulation of calcium-induced differentiation by human keratinocytes.
引用
收藏
页码:777 / 781
页数:5
相关论文
共 28 条