ENDOTHELIN-1 INDUCES PROSTACYCLIN RELEASE FROM BOVINE AORTIC ENDOTHELIAL-CELLS

The effects of endothelin-1 (ET-1) on the release of prostacyclin from cultured bovine aortic endothelial cells were studied. ET-1 induced a time- and dose-dependent release of 6-keto PGF1α, the stable metabolite of prostacyclin, with an apparent EC50 value of 3.0 ± 0.9 nM (n=6). ET-1 up to a concentration of 500 nM did not affect cellular integrity. Preincubation of the cells for 30 min with 10 μM indomethacin inhibited ET-1 (100 nM) - induced prostacyclin release by 90 %. These findings indicate that ET-1 can directly stimulate prostacyclin release from endothelial cells probably through a receptor mediated mechanism. © 1991.