CHARACTERIZATION OF MUSCARINIC ACETYLCHOLINE-RECEPTORS ON THE RAT PANCREATIC GASTRIN-PRODUCING CELL-LINE B6 RIN

被引：4

作者：

ABELLO, J ^{[1
]}

ROCHE, C ^{[1
]}

CUBER, JC ^{[1
]}

BERNARD, C ^{[1
]}

PHILIPPE, J ^{[1
]}

CHAYVIALLE, JA ^{[1
]}

机构：

[1] UNIV GENEVA,MED CTR,MICROBIOL LAB,CH-1211 GENEVA 4,SWITZERLAND

来源：

FEBS LETTERS | 1990年 / 270卷 / 1-2期

关键词：

B6 RIN cell; Carbachol; Cytosolic free calcium; Gastrin release; Muscarinic acetylcholine receptor;

D O I：

10.1016/0014-5793(90)81229-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The mechanisms of cholinergic stimulation of gastrin cells were studied in the rat pancreatic cell line B6 RIN. Carbachol induced an increase in intracellular Ca2+ and stimulated gastrin release in a dose-dependent manner over the range 10-5-10-3 M. These effects were completely abolished by atropine, suggesting the implication of muscarinic cholinergic receptors. The binding properties of these receptors were investigated. [N-Methyl-3H]scopolamine ([3h]nms) binding on cell homogenates was time-dependent, saturable and consistent with a single high-affinity binding class (Kd = 39.5 pM, and Bmax = 7.9 fmol mg DNA). Carbachol competitively inhibited [3H]NMS binding. The potency of inhibition of [3H]NMS binding by subtype selective antagonists was hexahydrodifenidol > pirenzepine > AF-DX 116. These results suggest the M3, muscarinic receptors may be involved in the carbachol-induced gastrin release from B6 RIN cells. © 1990.

引用

页码：37 / 40

页数：4

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