CHEMILUMINESCENCE ANALYSIS OF THE ACTIVITY OF PECTEN-MAXIMUS HEMOCYTES STIMULATED WITH ZYMOSAN AND HOST-SPECIFIC RICKETTSIALES-LIKE ORGANISMS

被引：49

作者：

LEGALL, G ^{[1
]}

BACHERE, E ^{[1
]}

MIALHE, E ^{[1
]}

机构：

[1] IFREMER,BIOL & ECOL INVERTEBRES MARINS LAB,UNITE RECH PATHOL IMMUNOL & GENET MOLEC,BP 133,F-17390 LA TREMBLADE,FRANCE

来源：

DISEASES OF AQUATIC ORGANISMS | 1991年 / 11卷 / 03期

关键词：

D O I：

10.3354/dao011181

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

During a study of Rickettsiales-like organisms (RLO) in Pecten maximus gill, histological observation of free RLO in hemolymph led us to consider interactions between the pathogen and the hemocytes. Firstly, the production of oxygen radicals by hemocytes was established using a luminol-enhanced chemiluminescence method with zymosan as stimulant. The effects of several inhibitors of oxidative metabolism were investigated. The internalization of RLO into hemocytes was demonstrated by electron microscopy but was not related to chemiluminescent activity. The possible involvement of RLO acid phosphatase as the inhibitor of respiratory burst was assessed.

引用

页码：181 / 186

页数：6