EFFECT OF TIME OF MATING RELATIVE TO OVULATION ON MORPHOLOGICAL DIVERSITY OF SWINE BLASTOCYSTS

The effect of time of mating relative to ovulation on the amount of embryonic diversity was investigated in 37 mature crossbred gilts. Ovulation was induced by injecting proestrous gilts with 1000 IU of hCG. Gilts were naturally mated once at 24-32 (control), 41, or 43 h after hCG injection (n = 10, 9, and 18. respectively). Blastocysts were collected surgically 288 h post-hCG from gilts mated at 24-32 h post-hCG, from those mated at 41 h post-hCG, and from 8 of those mated at 43 h post-hCG. Collection of blastocysts was performed at 295 h in 10 gilts mated at 43 h post-hCG. Blastocysts within the ranges 1.0-4.0, 4.5-7.0, 7.5-10.0, 10.5-15.0, and 15.5-40.0 mm and those > 40.0 mm were classified morphologically as small, medium, and large spheres and as ovoidal, tubular, and filamentous, respectively. Blastocysts were incubated for 6 h to quantify secretion of estradiol-17beta (estradiol). Morphological diversity (standard deviation of size) did not differ among blastocysts of treatment groups. Delaying the time of mating reduced (p < 0.07) the subsequent size and estradiol secretion of blastocysts. Waiting until 295 h after hCG to evaluate blastocysts of gilts mated at 43 h resulted in blastocyst size and estradiol secretion similar to that of concepti of controls. Estradiol secretion by medium and large spherical blastocysts of gilts mated at 43 h post-hCG and evaluated at 288 h was lower (p < 0.05) than that by blastocysts of the same classifications from controls. Delaying mating, relative to ovulation, decreased size and estradiol secretion of blastocysts but did not affect the extent of their morphological diversity.