MODULATED EXPRESSION OF PROMOTERS CONTAINING UPSTREAM CURVED DNA-SEQUENCES BY THE ESCHERICHIA-COLI NUCLEOID PROTEIN H-NS

Replacement of the CRP-binding site of the gal control region by curved sequences can lead to the restoration of promoter strength in vivo. One curved sequence balled 5A6A, however, failed to do so. The gene hns exerts a strong negative control on the resulting 5A6A gal promoter as well as on the distant bla promoter, specifically in a 5A6A gal context. The product of this gene, H-NS, displays a better affinity far this particular insert compared to other curved sequences. Mechanisms by which H-NS may repress promoters both at short and long distances from a favoured binding site. are discussed.