ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY AND HPLC DETERMINATION OF THE PRODUCTS IN THE RADIOLYSIS OF 5-BROMOURACIL, ITS NUCLEOSIDE AND NUCLEOTIDE DERIVATIVES

被引:10
作者
YE, MY [1 ]
SHEN, Y [1 ]
机构
[1] PACIFIC NW LAB, DEPT BIOL & CHEM, RICHLAND, WA 99352 USA
来源
JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 04期
关键词
D O I
10.1080/10826079408013368
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Radiolysis of 5-bromouracil (BrUr), 5-bromo-2'-deoxyuridine (BrdU) and 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdUMP) has been studied by high performance liquid chromatography and electrospray ionization mass spectrometry. Production of bromide ions and the complementary uracil, 2-deoxyuridine and 2-deoxyuridine-5'-monophosphate has been quantitatively examined. It is concluded that in the presence of hydroxyl radical scavenger, at pH 6 to 10, BrUr, BrdU and BrdUMP quantitatively react with hydrated electron. In the absence of hydroxyl radical (OH) scavenger in N-2-saturated solutions the yield of bromide ions is about 5.0, indicating that hydroxyl radicals also significantly react with BrUr, BrdU and BrdUMP to eliminate hydrogen bromide. Results from the radiolysis of 1 mM aqueous solutions of BrdU and BrdUMP suggested that abstraction of hydrogen by uracilyl radicals from the sugar moieties is not important. Our mass spectrometry experiments indicated that uracilyl radicals react with the starting substrate to form dimers. The radiolysis of N-2-saturated solutions of nucleosides and nucleotides suggested that the base radicals formed by OH addition can react with undamaged reagents to produce dimers.
引用
收藏
页码:773 / 791
页数:19
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