VIABILITY OF CLONED BOVINE EMBRYOS AFTER ONE OR 2 CYCLES OF NUCLEAR TRANSFER AND IN-VITRO CULTURE

被引：16

作者：

ECTORS, FJ ^{[1
]}

DELVAL, A ^{[1
]}

SMITH, LC ^{[1
]}

TOUATI, K ^{[1
]}

REMY, B ^{[1
]}

BECKERS, JF ^{[1
]}

ECTORS, F ^{[1
]}

机构：

[1] UNIV MONTREAL,FAC MED VET,CTR RECH & REPROD ANIM,ST HYACINTHE,PQ J2S 7C6,CANADA

来源：

THERIOGENOLOGY | 1995年 / 44卷 / 07期

关键词：

NUCLEAR TRANSFER; BOVINE; EMBRYO; IN VITRO CULTURE; IN VITRO FERTILIZATION;

D O I：

10.1016/0093-691X(95)00280-L

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We described an exclusively in vitro procedure for cloning and recloning bovine embryos. Embryos obtained by IVM/IVF/IVC developed to the morula stage were used as blastomere donors in cunjunction with IVM recipient oocytes. Reconstructed embryos were developed in vitro in coculture using bovine oviductal epithelial cells. The resulting morulae were used as donors for recloning under the same experimental conditions. No significant difference was observed between cloning and recloning in terms of development (rates of blastocysts: 12.9 versus 14.9%), in the number of nuclei per blastocyst (63.8 versus 49.1), or in pregnancy rates (35.7 versus 33.3%). The high variability observed between replicates and the correlation between results in first and second cycle nuclear transfer may suggest an inherant potential of individual donor embryos to support development by cloning.

引用

页码：925 / 933

页数：9

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