ENHANCED SHIKONIN PRODUCTION FROM LITHOSPERMUM-ERYTHRORHIZON BY INSITU EXTRACTION AND CALCIUM ALGINATE IMMOBILIZATION

被引：66

作者：

KIM, DJ ^{[1
]}

CHANG, HN ^{[1
]}

机构：

[1] KOREA ADV INST SCI & TECHNOL,DEPT CHEM ENGN,POB 131,SEOUL 131,SOUTH KOREA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1990年 / 36卷 / 05期

关键词：

D O I：

10.1002/bit.260360505

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plant cell cultures of Lithospermum erythrorhizon were carried out to produce shikonin by in situ extraction and cell immobilization in calcium alginate bead in shake flask cultures. In situ product extraction and cell immobilization enhanced shikonin production and facilitated product recovery. In situ extraction by n‐hexadecane and cell immobilization by calcium alginate gave higher specific shikonin productivities of 7.4 and 2.5 times, respectively, than those from the cultures of free cells without extraction. Simultaneous use of both techniques increased specific and volumetric productivities of shikonin 25‐ and 15‐fold, respectively. In calcium alginate immobilized cell cultures, n‐hexadecane addition at an early stage (before 15 days) was effective for shikonin production, and solvent addition after 15 days of the culture significantly reduced shikonin production. Higher numbers of plant cell immobilized bead inoculation did not increase shikonin production and sucrose consumption. Most of the produced shikonin was dissolved in the solvent layer. Copyright © 1990 John Wiley & Sons, Inc.

引用

页码：460 / 466

页数：7

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