CONSTRUCTION, BACTERIAL EXPRESSION AND CHARACTERIZATION OF A BIFUNCTIONAL SINGLE-CHAIN ANTIBODY-PHOSPHATASE FUSION PROTEIN TARGETED TO THE HUMAN ERBB-2 RECEPTOR

被引：132

作者：

WELS, W ^{[1
]}

HARWERTH, IM ^{[1
]}

ZWICKL, M ^{[1
]}

HARDMAN, N ^{[1
]}

GRONER, B ^{[1
]}

HYNES, NE ^{[1
]}

机构：

[1] CIBA GEIGY AG,DEPT MOLEC BIOL,BIOTECHNOL SECT,CH-4000 BASEL,SWITZERLAND

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 10期

关键词：

D O I：

10.1038/nbt1092-1128

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have constructed genes expressing single-chain antigen binding proteins (scFv) which recognize the human erbB-2 receptor. These genes encode the heavy and light chain variable domains of an erbB-2 receptor specific monoclonal antibody, MAb FRP5, connected by a peptide linker. In order to express a bifunctional molecule, a bacterial alkaline phosphatase gene was fused 3' to the scFv gene. The scFv(FRP5) and scFv(FRP5)-alkaline phosphatase fusion protein (scFv(FRP5)-PhoA) expressed in E. coli specifically recognize the human erbB-2 protein and compete with MAb FRP for binding to the receptor. The bound scFv(FRP5)-PhoA protein can be detected directly on tumor cells using a substrate for alkaline phosphatase, showing that the chimeric protein retains both binding and enzymatic activity.

引用

页码：1128 / 1132

页数：5

共 27 条

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