FLUORESCENT LABELING OF A DNA-SEQUENCING PRIMER

被引：6

作者：

MINENO, J ^{[1
]}

ISHINO, Y ^{[1
]}

OHMINAMI, T ^{[1
]}

KATO, I ^{[1
]}

机构：

[1] TAKARA SHUZO CO LTD,BIOTECHNOL RES LABS,OTSU,SHIGA 52021,JAPAN

来源：

DNA SEQUENCE | 1993年 / 4卷 / 03期

关键词：

DIDEOXY SEQUENCING; FLUORESCEIN; LABELING; PRIMER;

D O I：

10.3109/10425179309015626

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were hybridized with less stability than that containing fluorescein at only the 5' terminus but it formed more efficient primers for dideoxy sequencing with an automated sequencer.

引用

页码：135 / 141

页数：7

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