GROWTH-FACTORS AND CANINE FLEXOR TENDON HEALING - INITIAL STUDIES IN UNINJURED AND REPAIR MODELS

被引：82

作者：

DUFFY, FJ ^{[1
]}

SEILER, JG ^{[1
]}

GELBERMAN, RH ^{[1
]}

HERGRUETER, CA ^{[1
]}

机构：

[1] BRIGHAM & WOMENS HOSP,DIV PLAST SURG,BOSTON,MA 02115

来源：

JOURNAL OF HAND SURGERY-AMERICAN VOLUME | 1995年 / 20A卷 / 04期

关键词：

D O I：

10.1016/S0363-5023(05)80284-9

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

The role of growth factors in a variety of bone and soft tissue healing processes has been studied extensively in numerous recent models, yet little is known about the specific growth factors that may be playing a role in flexor tendon healing. We used a number of established protein purification techniques and bioassays to isolate and partially characterize a heparin-binding growth factor from unoperated canine tendons. Our data provide evidence that basic fibroblast growth factor, a potent angiogenic growth factor, is present in normal canine intrasynovial flexor tendons. We then studied repaired canine flexor tendons to further elucidate the role of growth factors in the tendon healing process. Heparin-sepharose elution profiles from three repair intervals (3, 10, and 17 days) were graphed and compared to known profiles of isolated growth factors. The three repair intervals demonstrated two elution profile peaks, consistent with varying amounts of platelet-derived growth factor and epidermal growth factor. Although additional experimentation is required to identify definitively the various protein isolates, these data provide compelling evidence that a variety of growth factors are present in uninjured and healing digital flexor tendons.

引用

页码：645 / 649

页数：5

共 22 条

[1]

Lobb, Sasse, Sullivan, Et al., Purification and characterization of heparin-binding endothelial cell growth factors, J Biol Chem, 261, pp. 1924-1928, (1986)

[2]

Gelberman, Khabie, Cahill, The revascularization of healing flexor tendons in the digital sheath, J Bone Joint Surg, 73 A, pp. 868-881, (1991)

[3]

Casscells, Speir, Sasse, Et al., Isolation, characterization, and localization of heparin-binding growth factors in the heart, 85, pp. 433-441, (1990)

[4]

Besner, Higashiyama, Klagsbrun, Isolation and characterization of a macrophage-derived heparin-binding growth factor, Cell Reg, 1, pp. 811-819, (1990)

[5]

Gelberman, Steinberg, Amiel, Ing, Akeson, Fibroblast chemotaxis after tendon repair, J Hand Surg, 16 A, pp. 686-693, (1991)

[6]

Folkman, Klagsbrun, Angiogenic factors, Science, 235, pp. 442-447, (1987)

[7]

Klagsbrun, Langer, Levenson, Smith, Lillehei, The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage, Exper Cell Res, 105, pp. 99-108, (1977)

[8]

Iberg, Rogelji, Fanning, Klagsbrun, Purification of 18 kDa forms of basic fibroblast growth factor from rat cells transformed by the Ras oncogene, J Biol Chem, 264, pp. 19951-19955, (1989)

[9]

Postlethwaite, Keski-oja, Balian, Kang, Induction of fibroblast chemotaxis by fibronectin, Journal of Experimental Medicine, 153, pp. 494-499, (1981)

[10]

Brigstock, Klagsbrun, Sasse, Farber, Iberg, Species-specific high molecular weight forms of basic fibroblast growth factor, Growth Factors, 4, pp. 45-52, (1990)

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