TRANSFORMATION OF THE YEAST KLUYVEROMYCES-LACTIS BY NEW VECTORS DERIVED FROM THE 1.6-MU-M CIRCULAR PLASMID PKD1

被引：120

作者：

BIANCHI, MM

FALCONE, C

CHEN, XJ

WESLOWSKILOUVEL, M

FRONTALI, L

FUKUHARA, H

机构：

[1] CTR UNIV ORSAY, INST CURIE, BIOL SECT, BATIMENT 110, F-91405 ORSAY, FRANCE

[2] UNIV ROME, DEPT CELLULAR & DEV BIOL, I-00185 ROME, ITALY

来源：

CURRENT GENETICS | 1987年 / 12卷 / 03期

关键词：

D O I：

10.1007/BF00436877

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The circular plasmid pKD1 (or 1.6 .mu.m DNA) has recently been isolated from Kluyveromyces drosophilarum. This plasmid appears to have a functional organization analogous to that of the 2 .mu. DNA of Saccharomyces cerevisiae, although the respective nucleotide sequences show little homology. pKD1 can be transferred to Kluyveromyces lactis where it is replicated stably. Using recombinant molecules derived from pKD1, a practical transformation system has been developed for Klyuveromyces lactis, with an efficiency and stability comparable to the 2 .mu.-based Saccharomyces cerevisiae transformation system.

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页码：185 / 192

页数：8

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