VIMENTIN IS HYPERPHOSPHORYLATED IN PRIMARY HUMAN FIBROBLASTS TREATED WITH OKADAIC ACID

被引：70

作者：

YATSUNAMI, J

FUJIKI, H

SUGANUMA, M

YOSHIZAWA, S

ERIKSSON, JE

OLSON, MOJ

GOLDMAN, RD

机构：

[1] NATL CANC CTR,RES INST,DIV CANC PREVENT,TOKYO 104,JAPAN

[2] UNIV MISSISSIPPI,MED CTR,DEPT BIOCHEM,JACKSON,MS 39216

[3] NORTHWESTERN UNIV,SCH MED,DEPT CELL MOLEC & STRUCT BIOL,CHICAGO,IL 60611

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 177卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90662-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Okadaic acid and dinophysistoxin-1 (35-methylokadaic acid) induced hyperphosphorylation of a 58 kDa protein in primary human fibroblasts, due to inhibition of protein phosphatase 1 and 2A activities. The protein was present in the nuclear and cytosolic fractions. Its pI was 5.3. The hyperphosphorylated protein reacted with monoclonal and polyclonal anti-vimentin antibodies, but not with anti-nucleolin antibody. Phosphorylation of vimentin was stimulated in vitro by dinophysistoxin-1 dose-dependently in the presence of protein phosphatase 2A and protein kinases. © 1991.

引用

页码：1165 / 1170

页数：6

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