CALCITONIN-GENE RELATED PEPTIDE IS AN EVOLUTIONARILY CONSERVED MARKER WITHIN THE AMNIOTE THALAMO-TELENCEPHALIC AUDITORY PATHWAY

The distribution of neurons and fibers containing calcitonin-gene-related peptide (CGRP) was mapped in the thalamo-telencephalic auditory pathways of four amniote species, rats, pigeons (Columba livia), caiman (Caiman crocodilus), and turtles (Pseudemys scripta). In colchicine-treated turtles and pigeons, numerous CGRP+ perikarya were observed in the auditory relay nucleus of the thalamus (n. reuniens of reptiles, and n. ovoidalis of birds). In pigeons, these neurons were most abundant in the outer circumference of the nucleus and were not observed without colchicine pretreatment. In the telencephalon of turtles, caiman, and pigeons, CGRP+ fibers were observed within portions of the dorsal ventricular ridge previously shown to receive projections from the auditory thalamus, thus implying that the thalamic CGRP+ neurons observed here in fact project to these telencephalic areas. In colchicine treated rats, numerous CGRP+ perikarya were observed along the ventral margin of the medial geniculate nucleus extending into the posterior intralaminar and peripeduncular nuclei, as well as occasionally within the ventral subdivision of the medial geniculate nucleus. Injections of fluorogold into the auditory cortex combined with immunofluorescence labeling for CGRP revealed that CGRP+ cells in these areas do, in fact, project to the auditory cortices. The present results are interpreted as providing strong support for the theory, advanced previously, that the medial geniculate nucleus of mammals, nucleus ovoidalis of birds, and nucleus reuniens of reptiles contain at least some homologous cell populations. Although the data are consistent with the theory that the telencephalic projection fields are homologous, other interpretations are also consistent with the data presented here. These include the possibility that auditory thalamic projections to the telencephalon arose independently in the lines of evolution leading to mammals and sauropsids.