STRUCTURAL CHARACTERIZATION OF THE 3'NONCODING REGION OF THE GENE ENCODING RAT MITOCHONDRIAL 3-HYDROXY-3-METHYLGLUTARYL COENZYME-A SYNTHASE

被引：7

作者：

AYTE, J ^{[1
]}

GILGOMEZ, G ^{[1
]}

HEGARDT, FG ^{[1
]}

机构：

[1] UNIV BARCELONA,FAC FARM,UNITAT BIOQUIM,DIAGONAL 643,E-08028 BARCELONA,SPAIN

来源：

GENE | 1993年 / 123卷 / 02期

关键词：

GENE CLONING; KETOGENESIS; MESSENGER RNA STABILITY; SECONDARY STRUCTURES; S1-NUCLEASE PROTECTION; POLYADENYLATION SIGNALS;

D O I：

10.1016/0378-1119(93)90136-Q

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We report the structural analysis of the 3' noncoding region of the rat mitochondrial 3-hydroxy-3-methylglutaryl coenzyme A synthase-encoding gene. This region, encoded by a single exon of the gene, spans 410 nucleotides. By using S1-nuclease protection analysis, we have mapped two major alternative polyadenylation signals, in close proximity. Computer analysis of the 3' noncoding region has detected the existence of four inverted repeats that could form stem-and-loop structures, with the thermodynamic free energy of formation ranging from -12.2 kcal/mol to -20.4 kcal/mol. The putative regulatory role of these structural features in mRNA stability is discussed.

引用

页码：267 / 270

页数：4

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