HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SEPARATION AND QUANTITATIVE ESTIMATION OF ANTIPARASITIC MELAMINOPHENYL ARSENICAL COMPOUNDS

被引：5

作者：

BERGER, BJ ^{[1
]}

FAIRLAMB, AH ^{[1
]}

机构：

[1] UNIV LONDON LONDON SCH HYG & TROP MED,DEPT MED PARASITOL,LONDON WC1E 7HT,ENGLAND

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1994年 / 88卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

10.1016/0035-9203(94)90117-1

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

A sensitive high-performance liquid chromatographic method has been developed for the separation and quantitative estimation of melaminophenyl arsenical drugs used in the treatment of human and veterinary trypanosomiases. Five clinically relevant compounds (melarsoprol, melarsonyl potassium, cymelarsan, melarsen oxide, and sodium melarsen) can be separated with an octadecylsilane reverse-phase column and detected down to 10 pmol per injection. The chromatographic system utilizes a propanol gradient in water, with lithium camphor sulphonate as ion modifier, and detection by ultraviolet absorbance at 280 nm. Preliminary results indicate that the arsenical compounds can be extracted and concentrated from serum using octadecylsilane solid-phase extraction cartridges. This method represents the first specific and sensitive assay system for separating and quantitatively estimating this important class of antiparasitic agents.

引用

页码：357 / 359

页数：3

共 14 条

[1]

BURRI C, 1992, TROP MED PARASITOL, V43, P223

[2] PHARMACOKINETIC PROPERTIES OF THE TRYPANOCIDAL DRUG MELARSOPROL [J].

BURRI, C ;

BALTZ, T ;

GIROUD, C ;

DOUA, F ;

WELKER, HA ;

BRUN, R .

CHEMOTHERAPY, 1993, 39 (04) :225-234

[3]

CRISTAU B., 1975, Medecine Tropicale, V35, P389

[4]

Cristau B, 1972, Med Trop (Mars), V32, P267

[5] THE INTERACTION OF ARSENICAL DRUGS WITH DIHYDROLIPOAMIDE AND DIHYDROLIPOAMIDE DEHYDROGENASE FROM ARSENICAL RESISTANT AND SENSITIVE STRAINS OF TRYPANOSOMA-BRUCEI-BRUCEI [J].

FAIRLAMB, AH ;

SMITH, K ;

HUNTER, KJ .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 53 (1-2) :223-231

[6] TRYPANOTHIONE IS THE PRIMARY TARGET FOR ARSENICAL DRUGS AGAINST AFRICAN TRYPANOSOMES [J].

FAIRLAMB, AH ;

HENDERSON, GB ;

CERAMI, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2607-2611

[7]

FRIEDHEIM EAH, 1949, AM J TROP MED, V29, P173

[8]

HAWKING FRANK, 1962, TRANS ROY SOC TROP MED AND HYG, V56, P354, DOI 10.1016/0035-9203(62)90004-4

[9]

JOHNSTONE RM, 1963, METABOLIC INHIBITORS, V2, P99

[10]

MAES L, 1988, ANN SOC BELG MED TR, V68, P219

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