DEXAMETHASONE SUPPRESSION OF IL-1-BETA-INDUCED CYCLOOXYGENASE-2 EXPRESSION IS NOT MEDIATED BY LIPOCORTIN-1 IN A549 CELLS

被引：38

作者：

NEWMAN, SP ^{[1
]}

FLOWER, RJ ^{[1
]}

CROXTALL, JD ^{[1
]}

机构：

[1] UNIV LONDON ST BARTHOLOMEWS HOSP & MED COLL,SCH MED,WILLIAM HARVEY RES INST,LONDON EC1M 6BQ,ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 202卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/bbrc.1994.2019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A549 cells grown under serum free conditions do not express cyclooxygenase 2 (cox 2). However, addition of IL-1 beta results in the induction of cox 2 in a time and dose dependent manner; actinomycin D completely blocks this induction. Dexamethasone completely suppressed the induction of cox 2 only if present during the first 3.5h of IL-1 beta treatment but not if added after 3.5h. Treatment with a neutralising monoclonal antibody (1A, Biogen) specific to lipocortin-1 failed to reverse this inhibition by dexamethasone. However, using this same antibody we were able to reverse completely the effects of dexamethasone on the suppression of PGE(2) release. These observations support the idea that glucocorticoids directly regulate cox 2 expression at the transcriptional level and this phenomenon is not not mediated by lipocortin-1. (C) 1994 Academic Press, Inc.

引用

页码：931 / 939

页数：9

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