STABILIZATION OF THE ESCHERICHIA-COLI ELONGATION-FACTOR TU-GTP-AMINOACYL-TRANSFER RNA COMPLEX

被引：12

作者：

DELARIA, K ^{[1
]}

GUILLEN, M ^{[1
]}

LOUIE, A ^{[1
]}

JURNAK, F ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE,DEPT BIOCHEM,RIVERSIDE,CA 92521

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1991年 / 286卷 / 01期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0003-9861(91)90029-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of ammonium sulfate on the Escherichia coli elongation factor Tu-GTP-aminoacyl-tRNA complex has been studied. The half-lives of 12 E. coli aminoacyl-tRNA species were determined at 37 °C in the presence and absence of an equimolar amount of EF-Tu-GTP and in the presence and absence of 1.5 m ammonium sulfate. The results indicate that the addition of 1.5 m ammonium sulfate to the ternary complex increased the stability of all 12 complexes studied. In addition, the effects of various salts and crystallization agents on the stability of the E. coli EF-Tu-GTP-phenylalanyl-tRNA complex was studied in detail. Binding parameters were also measured under various conditions at 37 °C. The results indicate that the stability and the Kassoc. of the ternary complex, using phenylalanyl-tRNA, can be increased by the presence of polyethylene glycol or ammonium sulfate. © 1991.

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页码：207 / 211

页数：5

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