PHENOTYPIC AND FUNCTIONAL-CHARACTERISTICS OF ACTIVATED CD8+ CELLS - A CD11B-CD28- SUBSET MEDIATES NONCYTOLYTIC FUNCTIONAL SUPPRESSION

被引:30
作者
FREEDMAN, MS
RUIJS, TCG
BLAIN, M
ANTEL, JP
机构
[1] Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montréal
来源
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1991年 / 60卷 / 02期
基金
英国医学研究理事会;
关键词
D O I
10.1016/0090-1229(91)90068-L
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Freshly isolated human CD8+ cells can be divided into mutually exclusive subsets bearing the phenotypes CD11b+(CD28-) or CD28+(CD11b-). We found that activation of CD8+ cells with anti-CD3 mAb and IL-2 preferentially expanded the CD11b-(CD28+) subset. This subset, when separated and activated independently, mediated both functional suppression and lectin-dependent cell cytotoxicity (LDCC). CD28- cells, prepared by elimination of the CD28+ cells from expanded unfractionated CD8+ cell cultures, retained functional suppressor activity but demonstrated reduced LDCC compared to either the CD28+(CD11b-)-enriched fraction or the unfractionated CD8+ population. The majority of the CD28- cells were also CD11b-, reflecting the observation that initially CD11b+ cells lose CD11b expression following activation with anti-CD3 mAb and IL-2. Our results therefore indicate that CD8+ cells deriving from the CD11b+CD28- subset, but expressing neither CD11b nor CD28 after activation, represent the main noncytotoxic functional suppressor cell in the mitogen "activated" suppressor assay. The preferential expansion of CD8+CD28+ cells relative to CD8+CD28- cells, if occuring in vivo in the central nervous system (CNS) compartment, would be consistent with observed phenotypic analysis of cerebrospinal fluid-derived T cells and might contribute to the reduced functional suppressor activity previously found for CNS compared to peripheral blood-derived lymphocytes. © 1991.
引用
收藏
页码:254 / 267
页数:14
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