FOLLICLE-STIMULATING-HORMONE ALONE SUPPORTS FOLLICLE GROWTH AND OOCYTE DEVELOPMENT IN GONADOTROPIN-RELEASING-HORMONE ANTAGONIST-TREATED MONKEYS

被引：155

作者：

ZELINSKIWOOTEN, MB

HUTCHISON, JS

HESS, DL

WOLF, DP

STOUFFER, RL

机构：

[1] OREGON REG PRIMATE RES CTR,BEAVERTON,OR 97006

[2] ARES ADV TECHNOL INC ARES SERONO,RANDOLPH,MA 02368

[3] OREGON HLTH SCI UNIV,DEPT PHYSIOL,PORTLAND,OR 97201

[4] OREGON HLTH SCI UNIV,DEPT OBSTET GYNECOL,PORTLAND,OR 97201

来源：

HUMAN REPRODUCTION | 1995年 / 10卷 / 07期

关键词：

FOLLICULAR STIMULATION; GNRH ANTAGONIST; IN-VITRO FERTILIZATION; RECOMBINANT HUMAN FSH; RECOMBINANT HUMAN LH;

D O I：

10.1093/oxfordjournals.humrep.a136151

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Both follicle stimulating hormone (FSH) and luteinizing hormone (LH) are proposed requirements for follicular growth and steroidogenesis; however, the role of LH in primate folliculogenesis is unclear. Follicular stimulation by recombinant human FSH (n = 5) with and without recombinant LH (1:1; n = 6) following 90 days of gonadotrophin-releasing hormone (GnRH) antagonist (Antide) treatment in macaques was evaluated. Human chorionic gonadotrophin (HCG) was administered when six follicles greater than or equal to 4 mm were observed. Oocytes were aspirated 27 h later and inseminated in vitro. Chronic Antide reduced serum oestradiol and bioactive LH to concentrations observed in hypophysectomized rhesus monkeys. Multiple follicular growth required a longer interval following recombinant FSH (12 +/- 1 days) than recombinant FSH + recombinant LH (9 +/- 0.2 days), but the total number of follicles/animal did not differ between groups. The day prior to HCG, oestradiol concentrations were 4-fold less following recombinant FSH compared to recombinant FSH + recombinant LH (12 and 52 +/- 11% respectively). Follicular growth and maturation in LH-deficient macaques occurred with FSH alone. Thus, LH is not required for folliculogenesis in primates. Higher fertilization rates following follicular stimulation with FSH alone suggest that the presence of LH with FSH (1:1) during the pre-ovulatory interval impairs gametogenic events in the periovulatory period.

引用

页码：1658 / 1666

页数：9

共 36 条

[1]

Chandrasekher Y., Brenner R.M., Molskness T.A., Yu Q., Stouffer R.L., Titrating luteinizing hormone (LH) surge requirements for ovulatory changes in primate follicles. II. Progesterone receptor expression in luteinizing granulosa cells, J. Clin. Endocrinol. Metab, 73, pp. 584-589, (1991)

[2]

Chandrasekher Y., Hutchison J.S., Zelinski-Wooten M.B., Hess D.L., Wolf D.P., Stouffer R.L., Initiation of periovulatory events in primate follicles using recombinant and native human luteinizing hormone to mimic the midcycle gonadotropin surge, J. Clin. Endocrinol. Metab, 79, pp. 298-306, (1994)

[3]

Bentick B., Shaw R.W., Iffland C.A., Burford G., Bernard A., A randomized comparative study of purified follicle stimulating hormone and human menopausal gonadotropin after pituitary desensitization with Buserelin for superovulation and in vitro fertilization, Fertil. Steril, 50, pp. 79-84, (1988)

[4]

Berger M.J., Taymor M.L., The role of luteinizing hormone in human follicular maturation and function, Am, J. Obstel. Gynecol, 111, pp. 708-710, (1971)

[5]

Chappel S.C., Howies C., Reevaluation of the roles of luteinizing hormone and follicle-stimulating hormone in the ovulatory process, Hum. Reprod, 6, pp. 1206-1212, (1991)

[6]

Couzinet B., Lestrat N., Brailly S., Forest M., Schaison G., Stimulation of ovarian follicular maturation with pure follicle-stimulating hormone in women with gonadotropin deficiency, J. Clin. Endocrinol. Metab, 66, pp. 552-556, (1988)

[7]

Edelstein M.C., Brzyski R.G., Jones G.S., Simonetti S., Muasher S.J., Equivalency of human menopausal gonadotropin and folliclestimulating hormone stimulation after gonadotropin-releasing hormone agonist suppression, Fertil. Steril, 53, pp. 103-106, (1990)

[8]

Ellinwood W.E., Resko J.A., Sex differences in Biologically active and immunoreactive gonadotropins in the fetal circulation of rhesus monkeys, Endocrinology, 107, pp. 902-907, (1980)

[9]

Galway A.B., Lapolt P.S., Tsafriri A., Dargan C.M., Boime I., Hsueh A., Recombinant follicle-stimulating hormone induces ovulation and tissue plasminogen activator expression in hypophysectomized rats, Endocrinology, 127, pp. 3023-3028, (1990)

[10]

Glasier A.F., Wickings E.J., Rodger M.W., Hillier S., Baird D., Both LH and FSH are required for the development of the normal follicle, J. Endocrinol, 119, (1988)

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