PHOTOCHEMICAL ENERGY-CONVERSION SYSTEM USING IMMOBILIZED CHLOROPLASTS

被引：15

作者：

KAYANO, H

MATSUNAGA, T

KARUBE, I

SUZUKI, S

机构：

[1] Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, Yokohama,227, Japan

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1981年 / 23卷 / 10期

关键词：

Agar gel - Clostridium butyricum - Conversion ratio - Energy conversion systems - Hydrogen-oxygen fuel cells - Optimum temperature - Phosphate buffer solutions - Photoinduced currents;

D O I：

10.1002/bit.260231011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Immobilized chloroplasts and Clostridium butyricum were employed for a photochemical energy conversion system. Spinach chloroplasts were immobilized in 2% agar gel. The optimum temperature of immobilized chloroplasts was 30°C. The maximum activity was obtained in the phosphate buffer solution (pH 8.0) containing 8μM of ferredoxin under an N2 bubbling condition. Hydrogen was evolved under illumination by immobilized chloroplasts and C. butyricum. Hydrogen produced by this system was applied to a hydrogen-oxygen fuel cell. Photoinduced current was obtained from this photochemical energy conversion system. A photocurrent of 0.4-1.5 mA was continuously obtained for 4 h. The conversion ratio from hydrogen to current was 80-100%. © 1981 John Wiley & Sons, Inc.

引用

页码：2283 / 2291

页数：9

共 12 条

[1] COPPER ENZYMES IN ISOLATED CHLOROPLASTS - POLYPHENOLOXIDASE IN BETA-VULGARIS [J].

ARNON, DI .

PLANT PHYSIOLOGY, 1949, 24 (01) :1-15

[2] HYDROGEN EVOLUTION BY A CHLOROPLAST-FERREDOXIN-HYDROGENASE SYSTEM [J].

BENEMANN, JR ;

BERENSON, JA ;

KAPLAN, NO ;

KAMEN, MD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (08) :2317-2320

[3] BIOELECTROCHEMICAL ENERGY CONVERSION [J].

BERK, RS ;

CANFIELD, JH .

APPLIED MICROBIOLOGY, 1964, 12 (01) :10-&

[4]

BROUN GB, 1979, ENZYME ENG, V6

[5]

DELDUCA MG, 1963, DEV IND MICROBIOL, V4, P81

[6] CONTINUOUS HYDROGEN PRODUCTION BY IMMOBILIZED WHOLE CELLS OF CLOSTRIDIUM-BUTYRICUM [J].

KARUBE, I ;

MATSUNAGA, T ;

TSURU, S ;

SUZUKI, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 444 (02) :338-343

[7] PHOTOCHEMICAL SYSTEM FOR REGENERATING NADPH FROM NADP WITH USE OF IMMOBILIZED CHLOROPLASTS [J].

KARUBE, I ;

OTSUKA, T ;

KAYANO, H ;

MATSUNAGA, T ;

SUZUKI, S .

BIOTECHNOLOGY AND BIOENGINEERING, 1980, 22 (12) :2655-2665

[8]

KARUBE I, 1979, BIOTECHNOL BIOENG, V21, P235

[9]

PIETRO AS, 1958, J BIOL CHEM, V231, P211

[10]

ROHRBACK GH, 1962, 16TH P ANN POW SOURC, P18

← 1 2 →