ORCOKININ - A NOVEL MYOTROPIC PEPTIDE FROM THE NERVOUS-SYSTEM OF THE CRAYFISH, ORCONECTES-LIMOSUS

被引：96

作者：

STANGIER, J

HILBICH, C

BURDZIK, S

KELLER, R

机构：

[1] UNIV BONN, INST ZOOPHYSIOL, ENDENICHER ALLEE 11-13, W-5300 BONN, GERMANY

[2] UNIV HEIDELBERG, ZENTRUM MOLEK BIOL, W-6900 HEIDELBERG, GERMANY

来源：

PEPTIDES | 1992年 / 13卷 / 05期

关键词：

MYOTROPIC NEUROPEPTIDE; CRUSTACEA; ORCONECTES-LIMOSUS; HINDGUT;

D O I：

10.1016/0196-9781(92)90041-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A myotropic peptide, named orcokinin, was isolated from approximately 1200 abdominal nerve cords of the crayfish, Orconectes limosus. Its amino acid sequence was determined as follows: Asn-Phe-Asp-Glu-Ile-Asp-Arg-Ser-Gly-Phe-Gly-Phe-Asn. This structure was confirmed by synthesis. There is no sequence similarity to any known neuropeptide. Orcokinin exhibits high potency on the crayfish hindgut, enhancing both frequency and amplitude of spontaneous contractions. The threshold of biological activity in vitro was determined to be approximately 5 x 10(-11) M.

引用

页码：859 / 864

页数：6

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