SOLUTION STRUCTURE OF OMEGA-CONOTOXIN MVIIA USING 2D NMR-SPECTROSCOPY

被引：64

作者：

BASUS, VJ ^{[1
]}

NADASDI, L ^{[1
]}

RAMACHANDRAN, J ^{[1
]}

MILJANICH, GP ^{[1
]}

机构：

[1] NEUREX CORP, MENLO PK, CA 94025 USA

来源：

FEBS LETTERS | 1995年 / 370卷 / 03期

基金：

美国国家科学基金会;

关键词：

NEUROTOXIN; CALCIUM CHANNEL BLOCKER; NMR STRUCTURE; COMPLETE RELAXATION MATRIX; CONUS MAGUS;

D O I：

10.1016/0014-5793(95)00819-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The solution structure of omega-conotoxin MVIIA (SNX-111), a peptide toxin from the fish hunting cone snail Conus magus and a high-affinity blocker of N-type calcium channels, was determined by 2D NMR spectroscopy. The backbones of the best 44 structures match with an average pairwise RMSD of 0.59 angstroms. The structures contain a short segment of triple-stranded beta-sheet involving residues 6-8, 20-21, and 24-25. The structure of this toxin is very similar to that of omega-conotoxin GVIA with which is has only 40% sequence homology, but very similar calcium channel binding affinity and selectivity.

引用

页码：163 / 169

页数：7

共 46 条

[1] BASUS VJ, 1989, METHOD ENZYMOL, V177, P132
[2] MLEV-17-BASED TWO-DIMENSIONAL HOMONUCLEAR MAGNETIZATION TRANSFER SPECTROSCOPY
BAX, A
DAVIS, DG
[J]. JOURNAL OF MAGNETIC RESONANCE, 1985, 65 (02) : 355 - 360
[3] BORGIAS BA, 1989, METHOD ENZYMOL, V176, P169
[4] MARDIGRAS - A PROCEDURE FOR MATRIX ANALYSIS OF RELAXATION FOR DISCERNING GEOMETRY OF AN AQUEOUS STRUCTURE
BORGIAS, BA
JAMES, TL
[J]. JOURNAL OF MAGNETIC RESONANCE, 1990, 87 (03): : 475 - 487
[5] SOLUTION STRUCTURE OF OMEGA-CONOTOXIN GVIA USING 2-D NMR-SPECTROSCOPY AND RELAXATION MATRIX ANALYSIS
DAVIS, JH
BRADLEY, EK
MILJANICH, GP
NADASDI, L
RAMACHANDRAN, J
BASUS, VJ
[J]. BIOCHEMISTRY, 1993, 32 (29) : 7396 - 7405
[6] STRUCTURAL DETERMINANTS OF THE BLOCKADE OF N-TYPE CALCIUM CHANNELS BY A PEPTIDE NEUROTOXIN
ELLINOR, PT
ZHANG, JF
HORNE, WA
TSIEN, RW
[J]. NATURE, 1994, 372 (6503) : 272 - 275
[7] SOLUTION STRUCTURE OF OMEGA-CONOTOXIN MVIIC, A HIGH-AFFINITY LIGAND OF P-TYPE CALCIUM CHANNELS, USING H-1-NMR SPECTROSCOPY AND COMPLETE RELAXATION MATRIX ANALYSIS
FARRJONES, S
MILJANICH, GP
NADASDI, L
RAMACHANDRAN, J
BASUS, VJ
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1995, 248 (01) : 106 - 124
[8] GRAY WR, 1988, ANNU REV BIOCHEM, V57, P665, DOI 10.1146/annurev.bi.57.070188.003313
[9] TWO-DIMENSIONAL CORRELATION OF CONNECTED NMR TRANSITIONS
GRIESINGER, C
SORENSEN, OW
ERNST, RR
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (22) : 6394 - 6396
[10] EFFECTS OF DISTANCE CONSTRAINTS ON MACROMOLECULAR CONFORMATION .2. SIMULATION OF EXPERIMENTAL RESULTS AND THEORETICAL PREDICTIONS
HAVEL, TF
CRIPPEN, GM
KUNTZ, ID
[J]. BIOPOLYMERS, 1979, 18 (01) : 73 - 81

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