MOLECULAR ANALYSIS OF AN AESCULINASE ACTIVITY FROM KLEBSIELLA-OXYTOCA

被引：1

作者：

ALZAAG, A

PEMBERTON, JM

机构：

[1] Department of Microbiology, University of Queensland, St Lucia,QLD,4067, Australia

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1988年 / 6卷 / 01期

关键词：

Cloning - Escherichia coli - Gene encoding - Salmonella;

D O I：

10.1111/j.1472-765X.1988.tb01203.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A DNA fragment containing a Klebsiella oxytoca gene for aesculinase activity was cloned on the multicopy plasmid pBR322. This β-glucosidase activity was confined to aesculin hydrolysis only. It was expressed equally well in Escherichia coli, Salmonella typhimurium and Aeromonas hydrophila. Two polypeptides were found to be encoded within the 2-6 kb of the cloned DNA encoding aesculinase activity. © 1988 Oxford University Press. All rights reserved.

引用

页码：11 / 13

页数：3

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