BEHAVIORAL-EFFECT OF ENGINEERED CELLS THAT SYNTHESIZE L-DOPA OR DOPAMINE AFTER GRAFTING INTO THE RAT NEOSTRIATUM

被引：86

作者：

HORELLOU, P

MARLIER, L

PRIVAT, A

MALLET, J

机构：

[1] CNRS, NEUROBIOL CELLULAIRE & MOLEC LAB, 1 ALLE TERRASSE, F-91198 GIF SUR YVETTE, FRANCE

[2] INSERM, U249, INST BIOL, F-30406 MONTPELLIER, FRANCE

来源：

EUROPEAN JOURNAL OF NEUROSCIENCE | 1990年 / 2卷 / 01期

关键词：

Cyclosporin A; graft; retroviral vector; transfection; tyrosine hydroxylase;

D O I：

10.1111/j.1460-9568.1990.tb00389.x

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Cell lines in which tyrosine hydroxylase was introduced either by infection or transfection were used in grafting experiments in a rat model of Parkinson's disease obtained by unilateral lesion of the substantia nigra. A neuroblastoma NS20 Y cell line which synthesizes only L‐DOPA and a neuroendocrine AtT‐20 cell line which produces dopamine were obtained. They were grafted into denervated striata and their ability to compensate for the dopaminergic deficit was studied. Both modified cell types displayed a rapid partial reversal of apomorphine‐induced turning behaviour. No effect was observed with the control unmodified cell lines. We discuss the usefulness of engineered cell lines to address the fundamental issues in grafting experiments and more particularly in the therapy of Parkinson's disease. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：116 / 119

页数：4

共 21 条

[1] CHOLECYSTOKININ - CORELEASE WITH DOPAMINE FROM NIGROSTRIATAL NEURONS IN THE CAT [J].