ESTIMATION OF PROTEIN FRACTIONAL SYNTHETIC RATE FROM TRACER DATA

被引：42

作者：

TOFFOLO, G

FOSTER, DM

COBELLI, C

机构：

[1] UNIV PADUA, DEPT ELECTR & INFORMAT, VIA GRADENIGO 6-A, I-35131 PADUA, ITALY

[2] UNIV WASHINGTON, CTR BIOENGN, SEATTLE, WA 98195 USA

来源：

AMERICAN JOURNAL OF PHYSIOLOGY | 1993年 / 264卷 / 01期

关键词：

RADIOACTIVE ISOTOPE; STABLE ISOTOPE; KINETICS; PROTEIN TURNOVER; MATHEMATICAL MODEL;

D O I：

10.1152/ajpendo.1993.264.1.E128

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

The fractional synthetic rate (FSR) is a key parameter characterizing protein turnover that is estimated from tracer kinetic data. Formulas to estimate this parameter usually assume a precursor-product model. Assuming this model is correct, we discuss these formulas to estimate the FSR in the steady and non-steady state both for the radioactive and stable isotope tracer. Then we deal with the non-steady-state case where the FSR becomes time varying and derive formulas for its estimation. A non-steady-state case study on the flooding-dose technique for measuring protein turnover is presented.

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页码：E128 / E135

页数：8

相关论文

共 17 条

[1] INCREASE IN ANTERIOR TIBIALIS MUSCLE PROTEIN-SYNTHESIS IN HEALTHY MAN DURING MIXED AMINO-ACID INFUSION - STUDIES OF INCORPORATION OF [1-C-13]LEUCINE [J].

BENNET, WM ;

CONNACHER, AA ;

SCRIMGEOUR, CM ;

SMITH, K ;

RENNIE, MJ .

CLINICAL SCIENCE, 1989, 76 (04) :447-454

[2] INTRINSICALLY DIFFICULT PROBLEMS - THE KINETICS OF BODY PROTEINS AND AMINO-ACIDS IN MAN [J].

BIER, DM .

DIABETES-METABOLISM REVIEWS, 1989, 5 (02) :111-132

[3] EFFECT OF EXERCISE AND RECOVERY ON MUSCLE PROTEIN-SYNTHESIS IN HUMAN-SUBJECTS [J].

CARRARO, F ;

STUART, CA ;

HARTL, WH ;

ROSENBLATT, J ;

WOLFE, RR .

AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (04) :E470-E476

[4] WHOLE-BODY AND PLASMA-PROTEIN SYNTHESIS IN EXERCISE AND RECOVERY IN HUMAN-SUBJECTS [J].

CARRARO, F ;

HARTL, WH ;

STUART, CA ;

LAYMAN, DK ;

JAHOOR, F ;

WOLFE, RR .

AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (05) :E821-E831

[5] COMPARTMENTAL MODEL OF LEUCINE KINETICS IN HUMANS [J].

COBELLI, C ;

SACCOMANI, MP ;

TESSARI, P ;

BIOLO, G ;

LUZI, L ;

MATTHEWS, DE .

AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 261 (04) :E539-E550

[6] TRACER-TO-TRACEE RATIO FOR ANALYSIS OF STABLE ISOTOPE TRACER DATA - LINK WITH RADIOACTIVE KINETIC FORMALISM [J].

COBELLI, C ;

TOFFOLO, G ;

FOSTER, DM .

AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 262 (06) :E968-E975

[7] CONSTANT SPECIFIC ACTIVITY INPUT ALLOWS RECONSTRUCTION OF ENDOGENOUS GLUCOSE-CONCENTRATION IN NONSTEADY STATE [J].

COBELLI, C ;

TOFFOLO, G .

AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (06) :E1037-E1040

[8]

CRYER DR, 1986, J LIPID RES, V27, P508

[9] DIFFERENTIAL-EFFECTS OF INSULIN DEFICIENCY ON ALBUMIN AND FIBRINOGEN SYNTHESIS IN HUMANS [J].

DEFEO, P ;

GAISANO, MG ;

HAYMOND, MW .

JOURNAL OF CLINICAL INVESTIGATION, 1991, 88 (03) :833-840

[10] MEASUREMENT OF THE RATE OF PROTEIN-SYNTHESIS IN MUSCLE OF POSTABSORPTIVE YOUNG MEN BY INJECTION OF A FLOODING DOSE OF [1-C-13]LEUCINE [J].

GARLICK, PJ ;

WERNERMAN, J ;

MCNURLAN, MA ;

ESSEN, P ;

LOBLEY, GE ;

MILNE, E ;

CALDER, GA ;

VINNARS, E .

CLINICAL SCIENCE, 1989, 77 (03) :329-336