EFFECT OF 3 CONCENTRATION TECHNIQUES ON VIABILITY OF CRYPTOSPORIDIUM-PARVUM OOCYSTS RECOVERED FROM BOVINE FECES

被引：97

作者：

BUKHARI, Z

SMITH, HV

机构：

[1] STOBHILL NHS TRUST,SCOTTISH PARASITE DIAGNOST LAB,GLASGOW G21 3UW,LANARK,SCOTLAND

[2] UNIV STRATHCLYDE,DIV ENVIRONM HLTH,GLASGOW G1 1XQ,LANARK,SCOTLAND

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 10期

关键词：

D O I：

10.1128/JCM.33.10.2592-2595.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bovine fecal samples (1 g) negative for Cryptosporidium sp. oocysts were seeded with 7 x 10(4) Cryptosporidium parvum oocysts and purified by either water-ether concentration, sucrose density flotation, or zinc sulfate flotation to evaluate oocyst recovery. The effect of these purification techniques on the viability of recovered oocysts was also evaluated, Significantly higher numbers of seeded oocysts were recovered by water-ether concentration (recovery rate, 46 to 75%) than by sucrose density (24 to 65%) or zinc sulfate (22 to 41%) flotation methods. In addition, water-ether concentration did not exert a significant effect: on the viability of the population of oocysts recovered, whereas sucrose density flotation and zinc sulfate flotation selectively concentrated viable oocysts. The water-ether concentration procedure is recommended for use in epidemiological studies in which both oocyst enumeration and viability assessment are required.

引用

页码：2592 / 2595

页数：4

共 14 条

[1] FURTHER OBSERVATIONS ON FORMOL-ETHER CONCENTRATION TECHNIQUE FOR FAECAL PARASITES [J].

ALLEN, AVH ;

RIDLEY, DS .

JOURNAL OF CLINICAL PATHOLOGY, 1970, 23 (06) :545-&

[2]

Blewett D. A., 1988, The management and health of farmed deer . A Seminar in the CEC Programme of Coordination of Research in Animal Husbandry, held in Edinburgh, UK, on 10-11 December 1987., P43

[3]

BUKHARI Z, UNPUB

[4] VIABILITY OF CRYPTOSPORDIUM-PARVUM OOCYSTS - CORRELATION OF INVITRO EXCYSTATION WITH INCLUSION OR EXCLUSION OF FLUOROGENIC VITAL DYES [J].