LOWERED SUPEROXIDE-DISMUTASE IN HIGHLY METASTATIC B16 MELANOMA-CELLS

被引：28

作者：

KWEE, JK ^{[1
]}

MITIDIERI, E ^{[1
]}

AFFONSO, OR ^{[1
]}

机构：

[1] INST NACL CANC PRACA CRUZ,CTR PESQUISA BASICA,VERMELHA 23,BR-20230 RIO DE JANEIRO,RJ,BRAZIL

来源：

CANCER LETTERS | 1991年 / 57卷 / 03期

关键词：

SUPEROXIDE DISMUTASE; METASTATIC CELLS; MELANOMA B16;

D O I：

10.1016/0304-3835(91)90157-D

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Superoxide dismutases (SOD), which are enzymes scavenging the superoxide radical, were studied in two variant lines of the B16 melanoma: B16F1 with low metastatic potential and B16F10 with high metastatic potential. SOD activity was measured by a method utilizing reduction in the chemiluminescence of luminol. Using cell free extracts it was shown that the highly metastatic B16F10 cell line has a SOD activity lower (20.70 +/- 3.07) units/mg protein, n = 8, than that of the less metastatic B16F1 cell line (81.38 +/- 6.78) units/mg protein, n = 8. Acrylamide gel electrophoresis suggested that Mn-SOD activity is higher in B16F1 cells.

引用

页码：199 / 202

页数：4

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