INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE FROM SULFOLOBUS-SOLFATARICUS AS A MODEL FOR STUDYING THERMOSTABLE TIM-BARREL ENZYMES

被引：14

作者：

ANDREOTTI, G ^{[1
]}

TUTINO, ML ^{[1
]}

SANNIA, G ^{[1
]}

MARINO, G ^{[1
]}

CUBELLIS, MV ^{[1
]}

机构：

[1] UNIV NAPLES FEDERICO II, DEPT ORGAN & BIOL CHEM, I-80134 NAPLES, ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1994年 / 1208卷 / 02期

关键词：

INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE; ARCHAEA; TIM-BARREL; THERMOSTABILITY; (S-SOLFATARICUS);

D O I：

10.1016/0167-4838(94)90118-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Indole-3-glycerol-phosphate synthase, a thermophilic and thermostable enzyme from the archaeon Sulfolobus solfataricus, was purified and characterized. The sequence of the thermophilic enzyme was compared to the sequence of a homologous mesophilic enzyme from Escherichia coli. The secondary structure of the thermophilic enzyme was predicted taking into account the patterns of hydropathy, chain flexibility and amphipathicity and the CD spectrum. From this analysis it turned out that indole-3-glycerol-phosphate synthase from S. solfataricus can be considered a model for studying thermostable TIM-barrel enzymes. some peculiarities of the amino-acid sequence of indole-3-glycerol-phosphate synthase from S. solfataricus are discussed in relation to the thermostability of the enzyme.

引用

页码：310 / 315

页数：6

共 32 条

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