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UBIQUITIN - PREPARATIVE CHEMICAL SYNTHESIS, PURIFICATION AND CHARACTERIZATION
被引：8
作者：
OGUNJOBI, O
RAMAGE, R
机构：
关键词：
D O I：
10.1042/bst0181322
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
An improved total synthesis of ubiquitin has been achieved by the Fmoc/t-butyl solid-phase methodology using N(G)Pmc protection of the Arg residues [1]. Optimization of the purification protocol has offered the material in substantial quantity (90 mg) in an overall yield of 4.2%. Characterization of the isolated products has opened the way to the studies of the folding pathway and the preparation of structural analogues.
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页码：1322 / 1323
页数：2
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