LIGHT-SCATTERING OF PROTEINS AS A CRITERION FOR CRYSTALLIZATION

被引：86

作者：

ZULAUF, M

DARCY, A

机构：

[1] F. Hoffmann-La Roche Ltd., Pharmaceutical Research - New Technologies

来源：

JOURNAL OF CRYSTAL GROWTH | 1992年 / 122卷 / 1-4期

关键词：

D O I：

10.1016/0022-0248(92)90232-8

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

Light scattering is particularly sensitive for the detection of aggregates as the scattered intensity is proportional to the square of the molecular weight of the scattering molecules. We have found that proteins showing a tendency to form aggregates in dilute solution (and in the absence of precipitating agents) do not crystallize in the majority of cases. Thus detection of aggregates seems to indicate that crystallization will not be successful. Fifteen proteins have been studied using this method to determine the correlation between aggregation and crystallization.

引用

页码：102 / 106

页数：5