FLOW CYTOMETRIC ANALYSIS OF CONCANAVALIN-A BINDING TO ISOLATED GOLGI FRACTIONS FROM RAT-LIVER

被引：14

作者：

GUASCH, RM

GUERRI, C

OCONNOR, JE

机构：

[1] UNIV VALENCIA, FAC MED,CTR REFERENCIA IZASA COULTER,DEPT BIOQUIM, BLASCO IBANEZ 17, E-46010 VALENCIA, SPAIN

[2] FDN VALENCIANA INVEST BIOMED, INST INVEST CITOL, E-46010 VALENCIA, SPAIN

来源：

EXPERIMENTAL CELL RESEARCH | 1993年 / 207卷 / 01期

关键词：

D O I：

10.1006/excr.1993.1172

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Flow cytometry (FCM) has been used repeatedly to study lectin binding to whole cells. However, there are very few attempts to analyze glycoconjugates in isolated subcellular organelles. We have applied FCM to quantitate the specific binding of fluorescein-conjugated concanavalin A (FITC-Con A) to isolated cis and trans fractions of rat liver Golgi complex, the cell compartment involved in glycoprotein maturation and sorting. Our results show similar intensities of Con A-specific binding in the two fractions. Using this method we show a decreased FITC-Con A binding to both fractions in ethanol-treated rats, which is consistent to previous work on alcoholic effects on galactosyltransferase. The possible applications of this technique are discussed. © 1993 Academic Press, Inc.

引用

页码：136 / 141

页数：6

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