ULTRASENSITIVE IMMUNOASSAY TECHNIQUES

被引：35

作者：

KRICKA, LJ

机构：

[1] Department of Pathology, Laboratory Medicine, University of Pennsylvania, Philadelphia

来源：

CLINICAL BIOCHEMISTRY | 1993年 / 26卷 / 05期

关键词：

IMMUNOASSAY; CHEMILUMINESCENCE; BIOLUMINESCENCE; FLUORESCENCE;

D O I：

10.1016/0009-9120(93)90108-I

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Ultrasensitive detection of clinically important substances using assays based on ligand:binder interactions has revolutionized laboratory medicine. Various strategies have been perfected to push the analytical sensitivity of ligand:binder assays (e.g., immunoassay, blotting, nucleic acid hybridization assay) into the attomole and zeptomole region (10(-18)-10(-21) mol). These include the use of labels with amplifying properties (e.g., enzymes), multiple labeling, and cascade reactions. In addition a wide range of ultrasensitive luminescent detection reactions have been developed for conventional enzyme labels based on chemiluminescent, bioluminescent, and time-resolved fluorescent reactions.

引用

页码：325 / 331

页数：7

共 45 条

[1] CHEMILUMINESCENT ASSAY OF VARIOUS ENZYMES USING INDOXYL DERIVATIVES AS SUBSTRATE AND ITS APPLICATIONS TO ENZYME-IMMUNOASSAY AND DNA PROBE ASSAY [J].