PAPAIN IMMOBILIZATION STUDY IN ENZYMATIC-SYNTHESIS OF DIPEPTIDE GLY-PHE

被引：6

作者：

CAPELLAS, M ^{[1
]}

SERRA, P ^{[1
]}

BENAIGES, MD ^{[1
]}

CAMINAL, G ^{[1
]}

GONZALEZ, G ^{[1
]}

LOPEZSANTIN, J ^{[1
]}

机构：

[1] UNIV AUTONOMA BARCELONA,FAC CIENCIES,CSIC,INST ENGN BIOQUIM,UNITAT ENGN QUIM,E-08193 BARCELONA,SPAIN

来源：

BIOCATALYSIS | 1994年 / 11卷 / 04期

关键词：

PAPAIN IMMOBILIZATION; ENZYMATIC PEPTIDE SYNTHESIS; ORGANIC SOLVENTS;

D O I：

10.3109/10242429409008860

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Papain catalysed peptide synthesis was followed to determine the optimum conditions for adsorption and covalent binding immobilization methods. The synthesis of the dipeptide Gly-Phe was studied in two different reaction systems: a) For adsorbed papain, in an organic medium containing low water concentration. b) For covalently bound enzyme, in a two-liquid phase system, using trichloroethylene as organic phase. The amount of enzyme offered to the immobilization support and the pH of the immobilization procedure were the main parameters studied.

引用

页码：273 / 281

页数：9

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