DETECTION OF ANTIBODIES TO HELICOBACTER-PYLORI CELL-SURFACE ANTIGENS

Serum IgG antibodies of Helicobacter pylori were detected in single-dilution ELISA using glycine extracted material. Among 148 endoscopy patients 59% displayed antibodies; as expected, a higher occurrence (90% was found in patients with positive gastric culture for H. pylori than in culture negative patients (37% Among 68 blood donors the frequency of H. pylori antibodies was 28% In 73 children <15 years of age examined for unrelated disorders the occurrence was 4% By immunoblotting using the same extract, 3 prominent bands, 29K, 54K and 60K and several weak bands were identified. These were formed by 57% 92% and 65% respectively, of the ELISA positive patient sera. Comparing culture positive and negative patients, the 3 bands occurred more often among the culture positive subjects though between 18 and 61% of the sera from culture negative patients gave either of the bands. When comparing the glycine extracts of 4 different H. pylori strains with separate haemagglutinating patterns no differences in the position of the major bands emerged. By absorption experiments no immunological cross-reactivity with components of Escherichia coli, Klebsiella pneumoniae, Campylobacter jejuni or C. fetus was found. Thus, the glycine extract seemed specific for the detection of antibodies to H. pylori. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.