CD77 - AN ANTIGEN OF GERMINAL CENTER B-CELLS ENTERING APOPTOSIS

被引:171
作者
MANGENEY, M
RICHARD, Y
COULAUD, D
TURSZ, T
WIELS, J
机构
[1] INST GUSTAVE ROUSSY,MICROSCOPIE CELLULAIRE & MOLEC LAB,F-94805 VILLEJUIF,FRANCE
[2] INSERM,U131,F-94800 VILLEJUIF,FRANCE
关键词
D O I
10.1002/eji.1830210507
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have previously reported that a neutral glycolipid (globotriaosylceramide; Gb3) was specifically expressed on Burkitt's lymphoma cells and on a subset of germinal center tonsillar B lymphocytes. Recently the Gb3 molecule was recognized as a new B cell differentiation antigen and now defines the CD77 cluster. Here we report an extensive phenotypic and functional characterization of the tonsillar CD77+ B lymphocytes. These cells have a low buoyant density and are thus purified using a Percoll gradient. They express various B cell antigens such as CD19, CD20, CD21, CD22, and CD40, as well as the adhesion molecules LFA-1, LFA-3 and CD44. They are positive for surface IgM and negative for surface IgD. Although these results suggest a phenotype of activated B cells, the CD77+ cells are negative for the classical activation antigens: CD23 (the low-affinity Fc receptor for IgE), CD25 [the interleukin (IL) 2 receptor alpha-chain] and CD71 (the transferrin receptor). Proliferation and protein synthesis of CD77+ cells was measured after stimulation with a range of mitogens and IL. None of the agents tested are able to induce proliferation and protein synthesis with the exception of a combination of recombinant IL 4 plus anti-CD40 antibody. When examined by electron microscopy, CD77+ B lymphocytes present a morphology similar to that of cells undergoing programmed cell death, also called apoptosis (i.e. chromatin condensation, nuclear fragmentation, membrane blebbing). As shown by direct examination of DNA, these CD77+ cells are indeed in the process of apoptosis. Treatment of the CD77+ cells by recombinant IL 4 and anti-CD40 antibody prevents apoptosis. All these results suggest that the CD77 molecule defines a B lymphocyte maturation pathway, specific for germinal center, where the cells undergo programmed cell death.
引用
收藏
页码:1131 / 1140
页数:10
相关论文
共 71 条
[1]   HUMAN EMBRYONAL CARCINOMA-CELLS AND THEIR DIFFERENTIATION IN CULTURE [J].
ANDREWS, PW ;
FENDERSON, B ;
HAKOMORI, SI .
INTERNATIONAL JOURNAL OF ANDROLOGY, 1987, 10 (01) :95-104
[2]   LONG-TERM HUMAN B-CELL LINES DEPENDENT ON INTERLEUKIN-4 AND ANTIBODY TO CD40 [J].
BANCHEREAU, J ;
DEPAOLI, P ;
VALLE, A ;
GARCIA, E ;
ROUSSET, F .
SCIENCE, 1991, 251 (4989) :70-72
[3]  
BENHAMOU LE, 1990, EUR J IMMUNOL, V20, P1685
[4]   STAGES OF B-CELL DIFFERENTIATION IN HUMAN LYMPHOID-TISSUE [J].
BHAN, AK ;
NADLER, LM ;
STASHENKO, P ;
MCCLUSKEY, RT ;
SCHLOSSMAN, SF .
JOURNAL OF EXPERIMENTAL MEDICINE, 1981, 154 (03) :737-749
[5]   SOLUBLE CD23 IS RELEASED BY B-LYMPHOCYTES CYCLING IN RESPONSE TO INTERLEUKIN-4 AND ANTI-BP50 (CDW40) [J].
CAIRNS, J ;
FLORESROMO, L ;
MILLSUM, MJ ;
GUY, GR ;
GILLIS, S ;
LEDBETTER, JA ;
GORDON, J .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (03) :349-353
[6]   ROLE OF THE BP35 CELL-SURFACE POLYPEPTIDE IN HUMAN B-CELL ACTIVATION [J].
CLARK, EA ;
SHU, G ;
LEDBETTER, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (06) :1766-1770
[7]   ACTIVATION OF HUMAN B-CELLS MEDIATED THROUGH 2 DISTINCT CELL-SURFACE DIFFERENTIATION ANTIGENS, BP35 AND BP50 [J].
CLARK, EA ;
LEDBETTER, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (12) :4494-4498
[8]  
CLARK EA, 1989, J IMMUNOL, V143, P3873
[9]  
DELOSTOYOS J, 1989, BLOOD, V2, P751
[10]  
DORKEN B, 1989, LEUCOCYTE TYPING, V4, P118