SOMATIC RECOMBINATION OF HEAVY-CHAIN VARIABLE REGION TRANSGENES WITH THE ENDOGENOUS IMMUNOGLOBULIN HEAVY-CHAIN LOCUS IN MICE

被引：25

作者：

GIUSTI, AM ^{[1
]}

COFFEE, R ^{[1
]}

MANSER, T ^{[1
]}

机构：

[1] THOMAS JEFFERSON UNIV,JEFFERSON MED COLL,JEFFERSON CANC INST,DEPT MICROBIOL & IMMUNOL,PHILADELPHIA,PA 19107

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 21期

关键词：

TRANSGENIC MICE; ANTIBODY GENES;

D O I：

10.1073/pnas.89.21.10321

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transgenic lines of mice were derived by using plasmid constructs containing DNA encoding an antibody heavy chain variable-diversity-joining region (V(H)-D-J(H)) and various amounts of 5' and 3' flanking DNA but tacking any repetitive isotype switch (S) or constant (C) region DNA. Unexpectedly, many of the antibody V(H) regions expressed by B-cell hybridomas generated from immunized transgenic mice were found to be of transgenic origin. Further analyses showed that somatic events had generated hybrid genomic loci in the mice containing the transgenic V(H)-D-J(H) gene and plasmid sequences 5' of endogenous heavy chain C region genes. Thus, V(H)-D-J(H) transgenes lacking S and C region DNA can recombine with endogenous Igh DNA, leading to the expression of transgene-encoded antibody.

引用

页码：10321 / 10325

页数：5

共 26 条

[1] JOINING OF IMMUNOGLOBULIN HEAVY-CHAIN GENE SEGMENTS - IMPLICATIONS FROM A CHROMOSOME WITH EVIDENCE OF 3 D-JH FUSIONS [J].