LIPOAMIDE DEHYDROGENASE OF STAPHYLOCOCCUS-AUREUS - NUCLEOTIDE-SEQUENCE AND SEQUENCE-ANALYSIS

被引：14

作者：

HEMILA, H

机构：

[1] Institute of Biotechnology, University of Helsinki, Helsinki

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1129卷 / 01期

关键词：

LIPOAMIDE DEHYDROGENASE; PYRUVATE DEHYDROGENASE; RIBOSOME; MEMBRANE TRANSPORT; PDHD; (STAPHYLOCOCCUS-AUREUS);

D O I：

10.1016/0167-4781(91)90225-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A complex of four proteins was previously isolated from Staphylococcus aureus. The complex had a strong interaction with membrane bound ribosomes, which suggested that it may be involved in protein secretion. However, the complex was identified as pyruvate dehydrogenase (PDH), which disproved the direct role of the complex in protein secretion. Here we report the nucleotide sequence of the last gene of the S. aureus pyruvate dehydrogenase operon, pdhD, which encodes lipoamide dehydrogenase (LPD). The pdhD gene encodes a protein of 468 amino acids, with a molecular mass of 49.5 kDa. The protein is closely related to other lipoamide dehydrogenases from bacteria and eukaryotes. The possible role of membrane bound lipoamide dehydrogenase is briefly discussed.

引用

页码：119 / 123

页数：5

共 38 条

[1] IMMUNOLOGICAL CROSS-REACTION BETWEEN PROTEINS SUPPOSED TO BE INVOLVED IN PROTEIN SECRETION IN STAPHYLOCOCCUS-AUREUS AND BACILLUS-SUBTILIS [J].