THE VPX GENE OF SIMIAN IMMUNODEFICIENCY VIRUS FACILITATES EFFICIENT VIRAL REPLICATION IN FRESH LYMPHOCYTES AND MACROPHAGES

被引：111

作者：

YU, XF ^{[1
]}

YU, QC ^{[1
]}

ESSEX, M ^{[1
]}

LEE, TH ^{[1
]}

机构：

[1] HARVARD UNIV,SCH PUBL HLTH,DEPT CANC BIOL,665 HUNTINGTON AVE,BOSTON,MA 02115

来源：

JOURNAL OF VIROLOGY | 1991年 / 65卷 / 09期

关键词：

D O I：

10.1128/JVI.65.9.5088-5091.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

vpx is a unique open reading frame found in simian immunodeficiency virus (SIV) and human immunodeficiency virus type 2 (HIV-2) but not in HIV-1. It encodes a 12- to 16-kDa virion-associated protein. Although vpx is dispensable for viral replication in several established human lymphocyte cell lines, there is no consensus regarding whether this gene is required for efficient viral replication in freshly isolated lymphocytes. We report here that the vpx mutant of SIV(mac) exhibits different degrees of impairment from wild-type SIV(mac) in freshly isolated lymphocytes. This defect is more pronounced in macrophages from the same donors. Our findings suggest that vpx is required for efficient viral replication in fresh lymphocytes and macrophages.

引用

页码：5088 / 5091

页数：4

共 24 条

[1] SEQUENCE OF SIMIAN IMMUNODEFICIENCY VIRUS FROM MACAQUE AND ITS RELATIONSHIP TO OTHER HUMAN AND SIMIAN RETROVIRUSES
CHAKRABARTI, L
GUYADER, M
ALIZON, M
DANIEL, MD
DESROSIERS, RC
TIOLLAIS, P
SONIGO, P
[J]. NATURE, 1987, 328 (6130) : 543 - 547
[2] COHEN EA, 1990, J ACQ IMMUN DEF SYND, V3, P11
[3] ISOLATION OF T-CELL TROPIC HTLV-III-LIKE RETROVIRUS FROM MACAQUES
DANIEL, MD
LETVIN, NL
KING, NW
KANNAGI, M
SEHGAL, PK
HUNT, RD
KANKI, PJ
ESSEX, M
DESROSIERS, RC
[J]. SCIENCE, 1985, 228 (4704) : 1201 - 1204
[4] A HIGHLY DIVERGENT HIV-2-RELATED ISOLATE
DIETRICH, U
ADAMSKI, M
KREUTZ, R
SEIPP, A
KUHNEL, H
RUBSAMENWAIGMANN, H
[J]. NATURE, 1989, 342 (6252) : 948 - 950
[5] THE HUMAN IMMUNODEFICIENCY VIRUS TYPE-2 (HIV-2) CONTAINS A NOVEL GENE ENCODING A 16-KD PROTEIN ASSOCIATED WITH MATURE VIRIONS
FRANCHINI, G
RUSCHE, JR
OKEEFFE, TJ
WONGSTAAL, F
[J]. AIDS RESEARCH AND HUMAN RETROVIRUSES, 1988, 4 (04) : 243 - 250
[6] SEQUENCE OF SIMIAN IMMUNODEFICIENCY VIRUS FROM AFRICAN-GREEN MONKEY, A NEW MEMBER OF THE HIV/SIV GROUP
FUKASAWA, M
MIURA, T
HASEGAWA, A
MORIKAWA, S
TSUJIMOTO, H
MIKI, K
KITAMURA, T
HAYAMI, M
[J]. NATURE, 1988, 333 (6172) : 457 - 461
[7] ISOLATION OF A T-LYMPHOTROPIC RETROVIRUS FROM NATURALLY INFECTED SOOTY MANGABEY MONKEYS (CERCOCEBUS-ATYS)
FULTZ, PN
MCCLURE, HM
ANDERSON, DC
SWENSON, RB
ANAND, R
SRINIVASAN, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (14) : 5286 - 5290
[8] VPX MUTANTS OF HIV-2 ARE INFECTIOUS IN ESTABLISHED CELL-LINES BUT DISPLAY A SEVERE DEFECT IN PERIPHERAL-BLOOD LYMPHOCYTES
GUYADER, M
EMERMAN, M
MONTAGNIER, L
PEDEN, K
[J]. EMBO JOURNAL, 1989, 8 (04) : 1169 - 1175
[9] GENOME ORGANIZATION AND TRANSACTIVATION OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2
GUYADER, M
EMERMAN, M
SONIGO, P
CLAVEL, F
MONTAGNIER, L
ALIZON, M
[J]. NATURE, 1987, 326 (6114) : 662 - 669
[10] THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-2 VPR GENE IS ESSENTIAL FOR PRODUCTIVE INFECTION OF HUMAN MACROPHAGES
HATTORI, N
MICHAELS, F
FARGNOLI, K
MARCON, L
GALLO, RC
FRANCHINI, G
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (20) : 8080 - 8084

← 1 2 3 →