CHARACTERIZATION OF 2 RADIATION-INDUCED LESIONS FROM DNA - STUDIES USING NUCLEASE-P1

被引：10

作者：

MACCUBBIN, AE

EVANS, MS

BUDZINSKI, EE

WALLACE, JC

BOX, HC

机构：

[1] ROSWELL PK CANC INST,DEPT BIOPHYS,BUFFALO,NY 14263

[2] ROSWELL PK CANC INST,DEPT EXPTL THERAPEUT,BUFFALO,NY 14263

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1992年 / 61卷 / 06期

关键词：

D O I：

10.1080/09553009214551581

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Exposure of DNA oligomers to ionizing radiation in oxygenated solution reveals that thc two lesions formed in highest yield are the 8-hydroxy modification of guanine and the formamido remnant of thymine. The effect of these lesions on the hydrolysis of thc phosphodiester bond by nuclease P1 was studied. Whereas the 8-hydroxyguanine lesion does not affect hydrolysis of the adjacent 3' phosphoester bond by nuclease P1, the formamido lesion markedly retards hydrolysis. Consistent with the oligomer results, digestion of irradiated DNA polymer by nuclease P1 plus acid phosphatase yielded the 8-hydroxyguanine lesion, obtained as the modified nucleoside, and the formamido lesion, obtained as a modified dinucleotide.

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页码：729 / 736

页数：8

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