A UREASE-NEGATIVE MUTANT OF HELICOBACTER-PYLORI CONSTRUCTED BY ALLELIC EXCHANGE MUTAGENESIS LACKS THE ABILITY TO COLONIZE THE NUDE-MOUSE STOMACH

被引：244

作者：

TSUDA, M ^{[1
]}

KARITA, M ^{[1
]}

MORSHED, MG ^{[1
]}

OKITA, K ^{[1
]}

NAKAZAWA, T ^{[1
]}

机构：

[1] YAMAGUCHI UNIV,SCH MED,DEPT INTERNAL MED 1,UBE,YAMAGUCHI 755,JAPAN

来源：

INFECTION AND IMMUNITY | 1994年 / 62卷 / 08期

关键词：

D O I：

10.1128/IAI.62.8.3586-3589.1994

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The urease of Helicobacter pylori has been proposed to be one of its pathogenic factors. A kanamycin resistance determinant was inserted in a cloned urease gene, and transformation-mediated allelic exchange mutagenesis was carried out to introduce the disrupted gene into the corresponding wild-type chromosomal region of a clinical isolate of H. pylori, CPY3401. The resulting mutant, HPT73, had the null activity of urease. Nude mouse stomachs were challenged with these two isogenic strains to examine the role of urease in pathogenesis. Gastritis was found in the CPY3401-challenged stomachs, from which bacteria indistinguishable from CPY3401 were recovered. There was no gastritis in the HPT73-challenged stomachs, and we could not recover H. pylori from them. These results indicated that H. pylori urease is essential for colonizing the nude mouse stomach.

引用

页码：3586 / 3589

页数：4

共 19 条

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