IDENTIFICATION OF MYCOBACTERIA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：187

作者：

BUTLER, WR ^{[1
]}

JOST, KC ^{[1
]}

KILBURN, JO ^{[1
]}

机构：

[1] TEXAS DEPT HLTH,BUR LABS,DIV MICROBIOL SERV,AUSTIN,TX 78756

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 11期

关键词：

D O I：

10.1128/JCM.29.11.2468-2472.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycolic acids extracted from saponified mycobacterial cells were examined as p-bromophenacyl esters by high-performance liquid chromatography (HPLC). Standard HPLC patterns were developed for species of Mycobacterium by examination of strains from culture collections and other well-characterized isolates. Relative retention times of peaks and peak height comparisons were used to develop a differentiation scheme that was 98% accurate for the species examined. A rapid, cost-effective HPLC method which offers an alternative approach to the identification of mycobacteria is described.

引用

页码：2468 / 2472

页数：5

共 13 条

[1] BAESS I, 1979, ACTA PATH MICRO IM B, V87, P221
[2] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY PATTERNS OF MYCOLIC ACIDS AS CRITERIA FOR IDENTIFICATION OF MYCOBACTERIUM-CHELONAE, MYCOBACTERIUM-FORTUITUM, AND MYCOBACTERIUM-SMEGMATIS
BUTLER, WR
KILBURN, JO
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (09) : 2094 - 2098
[3] IDENTIFICATION OF MAJOR SLOWLY GROWING PATHOGENIC MYCOBACTERIA AND MYCOBACTERIUM-GORDONAE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF THEIR MYCOLIC ACIDS
BUTLER, WR
KILBURN, JO
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1988, 26 (01) : 50 - 53
[4] PHENACYL ESTERS OF FATTY-ACIDS VIA CROWN ETHER CATALYSTS FOR ENHANCED ULTRAVIOLET DETECTION IN LIQUID-CHROMATOGRAPHY
DURST, HD
MILANO, M
KIKTA, EJ
CONNELLY, SA
GRUSHKA, E
[J]. ANALYTICAL CHEMISTRY, 1975, 47 (11) : 1797 - 1801
[5] FLOYD MM, 1990, 90TH ANN M AM SOC MI, P146
[6] HEIFETS L, 1989, J CLIN MICROBIOL, V27, P229, DOI 10.1128/JCM.27.1.229-.1989
[7] DETERMINATION OF MOLECULAR-SPECIES COMPOSITION OF C80 OR LONGER-CHAIN ALPHA-MYCOLIC ACIDS IN MYCOBACTERIUM SPP BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY AND MASS CHROMATOGRAPHY
KANEDA, K
NAITO, S
IMAIZUMI, S
YANO, I
MIZUNO, S
TOMIYASU, I
BABA, T
KUSUNOSE, E
KUSUNOSE, M
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1986, 24 (06) : 1060 - 1070
[8] KENT PT, 1985, CDC868230 US DEP HLT, P721
[9] MYCOLIC ACID PATTERNS OF SOME SPECIES OF MYCOBACTERIUM
MINNIKIN, DE
MINNIKIN, SM
PARLETT, JH
GOODFELLOW, M
MAGNUSSON, M
[J]. ARCHIVES OF MICROBIOLOGY, 1984, 139 (2-3) : 225 - 231
[10] RAMOS LS, 1991, 42ND PITTSB C AN CHE

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